Transgenesis workshop in mice

Mouse Transgenesis Workshop

Transgenesis workshop in mice

 

Transgenesis activity in mice is developed within the MoDiT team. It is based on more than 20 years of methodological experience in this field and on the existence of an experimental facility at the Jouy-en-Josas centre dedicated to the breeding of these animals in a confined environment.

 

The production of transgenic models can consist of an overexpression of a gene, ectopic or not, its invalidation or its directed mutagenesis.

These three approaches are obtained by microinjection into mouse embryos at the unicellular stage of DNA (often in one of the two pro-nuclei), RNA (often cytoplasmic, sometimes nuclear) or lentiviral vectors (in the periyolk space).

It can sometimes occur later if, for example, a targeting of embryonic appendages is desired.

This activity is mainly developed within the framework of research carried out by the MoDiT team on tissue differentiation, within the framework of intra-unit collaboration or with external laboratories (INRAE, INSERM, CNRS, etc.).

Modification date : 24 October 2023 | Publication date : 17 February 2014 | Redactor : P. Huan